TransFix® - Bone Marrow Stabilisation
Bone marrow examination is an essential procedure for the evaluation of a variety of clinical haematologic disorders and for preclinical toxicity studies of anticancer compounds and immunosuppressive agents. The short processing and analysis time, and the ease with which batches of samples can be analysed, has resulted in bone marrow flow cytometry becoming a routine test in clinical laboratories. Research work is currently being performed on the stabilisation of bone marrow aspirates with TransFix® EDTA for up to 10 days following aspiration, allowing samples to be batched and transported prior to analysis.
Sample preparation for flow cytometry:
- Place between 3ml and 5ml of aspirated bone marrow into a tube and add 0.2ml of TransFix per ml of bone marrow, and mix by inversion 5-10 times.
- Store at 2 - 8°C for up to 10 days.
- Allow samples to return to room temperature prior to flow cytometry. Also ensure that cells are resuspended by inverting the vial 30x.
- Sample preparation is detailed in the protocol available in Resources.
TransFix® Sample Storage Tubes
|TF-01-2||2 x 1ml Sample Storage Tubes containing 0.2ml TransFix® - sample|
|TF-01-10||10 x 1ml Sample Storage Tubes containing 0.2ml TransFix®|
|TF-01-25||25 x 1ml Sample Storage Tubes containing 0.2ml TransFix®|
|TF-01-50||50 x 1ml Sample Storage Tubes containing 0.2ml TransFix®|
|TFB-01-1||1ml vial TransFix® Bulk - sample|
|TFB-20-1||20ml vial TransFix® Bulk|
|TFB-20-5||100ml TransFix® Bulk (supplied as 5 x 20ml)|
|TFB-100-1||100ml TransFix® Bulk (supplied as 1 x 100ml)|
Other formats of TransFix® are available - click here.