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Bibliography

Bibliography

Human Blood Stabilisation - Other

2016

Stabilization of Transfected Cells Expressing Low-Incidence Blood Group Antigens: Novel Methods Facilitating Their Use as Reagent-Cells

Sample Type: Cell lines expressing blood group antigens

Analysis Method: Morphological analysis

Authors

C. González, R. Esteban, C. Canals, E. Muñiz-Díaz, N. Nogués

Publication

PLoS ONE (2016) 11(9): e0161968

This paper aimed to generate cell lines expressing blood group antigens (to represent low incidence blood group phenotypes) and to then stabilise these to facilitate their use as reagent cells in clinical laboratories. The addition of TransFix to the cells allowed cell stabilisation and antigen detection for at least 120 days and may facilitate the use of these cells in clinical laboratories, overcoming the limited availability of rare red blood cells. TransFix treated cells were test at 11 time points from Day 0 up to Day 120 showing better morphological preservation (FSC & SSC) vs fresh than lyophilised cells.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161968

The Association of High Prevalence of Trophozoites in Peripheral Blood with Lower Antibody Response to P. falciparum Infected Erythrocytes among Asymptomatic Children in Sudan

Sample Type: Malaria infected erythrocytes

Analysis Method: Flow Cytometry

Authors

S. N. Mohamed, D. A. Hassan, A. M. El Hussein, I. M. Osman, M. E. Ibrahim, H. S. Mohamed, B. Y. Nour, N. H. Abdulhadi

Publication

Mediators of Inflammation Article ID 7987686

The Authors of this paper examined the antibody reactivity of autologous plasma from symptomatic and asymptomatic malaria infected children against the infected erythrocytes' surface antigens using flow cytometry. The Authors use TransFix to stabilise variant surface antigens (VSA) for flow cytometric analysis.

https://www.hindawi.com/journals/mi/2016/7987686/abs/

2015

CD26 Expression on T Helper Populations and sCD26 Serum Levels in Patients with Rheumatoid Arthritis

Sample Type: T helper cells

Analysis Method: Flow Cytometry

Authors

O. J. Cordero, R. Varela-Calviño, T. López-González, C. Calviño-Sampedro, J. E. Viñuela, C. Mouriño, I. Hernández-Rodríguez, M. Rodríguez-López, B. Aspe de la Iglesia, J. M. Pego

Publication

PloS One 10(7):e0131992

The Authors studied dipeptidyl peptidase IV (DPP-IV, CD26) expression in different T helper cells and serum soluble DPP-IV/sCD26 levels in rheumatoid arthritis (RA) patients, correlated these with disease activity score (DAS), and examined how they were affected by different therapies. The authors concluded that, according to their CD26 expression, different cell subsets could serve to monitor RA course, and an uncharacterized T helper CD26- subset, not targeted by therapies, should be monitored for early diagnosis. Blood cells were collected using TransFix Vacuum Blood Collection Tubes and stored at 4°C until use.

http://www.ncbi.nlm.nih.gov/pubmed/26177310

Platelet leukocyte aggregates and markers of platelet aggregation, immune activation and disease progression in HIV infected treatment naive asymptomatic individuals

Sample Type: Platelet monocyte and platelet neutrophil aggregates

Analysis Method: Flow Cytometry

Authors

B. B. Nkambule, G. Davison

Publication

Journal of Thrombosis and Thrombolysis, 40 (4): 458-467

The primary aim of this study was to determine the levels of platelet monocyte and platelet neutrophil interactions in HIV treatment naive individuals. This was achieved by measuring the levels of platelet monocyte and platelet neutrophil aggregates and assessing the association between platelet aggregates; markers of immune activation and disease progression. Measurement of Platelet CD62P (platelet activation marker), CD36 (platelet aggregation marker), PMAs (platelet monocyte aggregates, CD14+CD42b+), and PNAs (platelet neutrophil aggregates, CD16+CD42b+) was performed by flow cytometry. TransFix was added to whole blood samples at the time of red blood cell lysis to keep the consequential platelet activation to a minimum, limiting artefactual platelet activation induced by ADP which is released upon the lysis of red blood cells.

https://link.springer.com/article/10.1007/s11239-015-1212-8

Quantification of Cells with Specific Phenotypes I: Determination of CD4+ Cell Count Per Microliter in Reconstituted Lyophilised Human PBMC Prelabeled with Anti-CD4 FITC Antibody

Sample Type: Human peripheral blood mononuclear cells

Analysis Method: Flow Cytometry

Authors

Stebbings R, Wang L, Sutherland J, Kammel M, Gaigalas AK, John M, Roemer B, Kuhne M, Schneider RJ, Braun M, Engel A, Dikshit DK, Abbasi F, Marti GE, Sassi MP, Revel L, Kim SK, Baradez MO, Lekishvili T, Marshall D, Whitby L, Jing W, Ost V, Vonsky M, Neukammer J.

Publication

Cytometry Part A (2015): 87A: 244-253

A surface-labelled lyophilised lymphocyte (Sll) preparation has been developed using human peripheral blood mononuclear cells prelabelled with a fluorescin isothiocyanate conjugated anti-CD4 monoclonal antibody. The sLL preparation is intended to be used as a reference material for CD4+ counting, including the development of higher order reference measurement procedures and has been evaluated in the pilot study CCQM-P102. The sLL reference material was prepared from a pool of three buffy coats from normal human donors, and the PBMCs were separated and labelled with anti-CD4 FITC monoclonal antibody, washed and resuspended in a fixative solution comprised of 10% TransFix.

http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22614/abstract

2014

Enumeration of circulating fibrocytes for clinical use is asthma by oprimized single-platform flow cytometry assay

Sample Type: Circulating fibrocytes

Analysis Method: Flow Cytometry

Authors

L Bianchetti, M Isgro, MA Marini, M Schmidt

Publication

BBA Clinical, Volume 1, June 2014, Pages 52–58

Elevated numbers of circulating fibrocytes is emerging as an easily accessible biomarker for asthma. In this report, blood samples were taken from 44 patients with asthma of various severity and 14 age-matched healthy individuals. Fibrocytes were quantified using flow cytometry. Blood samples were either processed within four hours or mixed with the cellular surface antigen-stabilizing agent TransFix and stored at 4 degrees Celsius for up to 96 hours before processing. Robust intra-assay variability demonstrated a good agreement between the fibrocyte counts measured in fresh whole blood and the fibrocyte counts obtained in stored whole blood at 48 and 96h.

http://www.sciencedirect.com/science/article/pii/S2214647414000099

Evaluation of TransFix® mediated stabilisation of adipose-derived stromal vascular fraction for delayed flow cytometry analysis

Sample Type: Adipose-derived stromal vascular fraction

Analysis Method: Flow Cytometry

Authors

Karsten,E., Sung,J., Morgan,C., Herbert,B., Vesey,G.

Publication

Open Journal of Regenerative Medicine (2014), 3, 54-63

In multi-centre studies where stromal vascular fraction (SVF) is used for immediate treatment, post-hoc assessment at a central or core facility of cell numbers, cell viability and immunophenotype may be necessary. Additional time taken to ship and store these samples can lead to time-dependant loss of cell markers and a reduction in cell viability. This paper provides an assessment of the suitability of TransFix for the purpose of SVF stabilisation across 77 consenting patients. The data shows that following treatment with TransFix the samples remain stable for up to seven days providing accurate total nucleated cell counts, stable populations of CD90, CD31 and CD45 markers and the potential to back calculate cell viability in the original sample. The authors also report an apparent increase in cell numbers post-stabilisation that is hypothesised to be due to a reduction in cell clumping as a result of treatment with TransFix.

http://www.scirp.org/journal/PaperInformation.aspx?paperID=49498#.VLPTzCusWTI

The role of tissue factor in activation coagulation system at pregnancy complications

Sample Type: Monocytes

Analysis Method: Flow Cytometry

Authors

M. Prochazka, L. Slavik L, J. Prochazkova, J. Ulehlova, M. Novák, P. Polak, R. Pilka

Publication

Thrombosis Research, 133: S109

The authors of this poster abstract propose a model to monitor activation of the coagulation system in preeclampsia and other pregnancy complications with Tissue Factor (TF) expression on monocytes by ?ow cytometry, and simultaneously ?xing the TF-induced thrombin generation in plasma. To determine expression of tissue factor (CD142) on monocytes, the authors used multicolour ?ow cytometry (CD45, CD14, CD16b, and CD142), and used TransFix to stabilise samples that could not be analysed within 2 hours.

https://www.mendeley.com/research/role-tissue-factor-activation-coagulation-system-pregnancy-complications/

2013

Quantification of circulating CD34+/KDR+/CD45 dim endothelial progenitor cells: analytical considerations

Sample Type: Circulating endothelial progenitor cells

Analysis Method: Flow Cytometry

Authors

E. M. Van Craenenbroeck, A. H. Van Craenenbroeck, S. van Ierssel, L. Bruyndonckx, V. Y. Hoymans, C J. Vrints, V. M. Conraads

Publication

International Journal of Cardiology, 167 (5): 1688-1695

A comprehensive review of characterisation of circulating endothelial progenitor cells with a particular focus on the considerations surrounding enumeration by flow cytometry and their impact on cardiovascular disease. The authors note TransFix can be used to prolong sample storage time for 7 days for this type of analysis.

http://www.sciencedirect.com/science/article/pii/S0167527312014222

Relationships between emerging cardiovascular risk factors, z-BMI, waist circumference and body adiposity index (BAI) on adolescents

Sample Type: Circulating endothelial cells

Analysis Method: Flow Cytometry

Authors

I. B. F. Dias, D. G. Panazzolo,M. F. Marques, B. D. Paredes, M. G. C. Souza, D. P. Manhanini,V. Morandi, P. T. V. Farinatti, E. Bouskela, L. G. Kraemer-Aguiar

Publication

Clin Endocrinol (2013) 79(5): 667-674

The body adiposity index (BAI) has been recently proposed as an alternative index to body mass index (BMI) and waist circumference (WC) to evaluate adiposity in adults. This study aimed to determine the correlation between BAI and emerging cardiovascular risk markers including circulating endothelial cells (CECs) in normal weight and obese adolescents. Percentage of CECs in peripheral blood were assessed by fow cytometric analysis: 400 uL cellular stabilization solution (TransFix) was added to a 4 ml of peripheral blood to preserve cell surface antigens.

http://onlinelibrary.wiley.com/doi/10.1111/cen.12195/full


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