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Animal Blood Stabilisation


Vaccination Against Histomonosis Limits Pronounced Changes of B-cells and T-cell Subsets in Turkeys and Chickens

Sample Type: Chicken and Turkey T-cell and B-cell subsets

Analysis Method: Flow Cytometry


T. Mitraa, W. Gernerb, F. A. Kidanea, P. Wernsdorfa, M. Hessa, A. Saalmüllerb, D. Liebharta


Vaccine (2017) 35(33): 4184-4196

The protozoan parasite histomonas is causative agent of histomonosis in Turkeys and chickens and causes a high mortality rate in Turkeys. Experimental vaccination has shown to protect these species. TransFix was used to stabilise 0.75ml and 1ml whole blood samples in order to analyse monocytes/macrophages and heterophils via Flow cytometry.


KEY PAPER: A rapid high-precision flow cytometry based technique for total white blood cell counting in chickens

Sample Type: Chicken White Blood Cell

Analysis Method: Flow Cytometry


C.Seliger, B. Schaerer, M. Kohn, H. Pendl, S. Weigend, B.Kaspers, S. Härtle


Veterinary Immunology and Immunopathology, Volume 145, pages 86–99, 2012

TransFix (Cytomark) was used to stabilise samples to demonstrate that samples can be shipped and stored prior to analysis. TransFix was used to stabilise and transport the avian blood samples. The automated analysis of total white blood cell count and white blood cell differentials is routine in research and clinical diagnosis in mammalian species. In contrast, in avian haematology these parameters are still estimated by conventional microscopic procedures due to technical difficulties associated with the morphological peculiarities of avian erythrocytes and thrombocytes. Both cell types are nucleated and fairly resistant to cell lysis, a prerequisite for automated leukocyte quantification and differentiation by commercial instruments. By using an anti-CD45 monoclonal antibody in combination with selected subset specific markers we have established a simple (no-lyse no-wash single-step one-tube) flow cytometry based technique for high precision chicken blood cell quantification.


KEY PAPER: Effects of Cyclosporin A induced T-lymphocyte depletion on the course of avian Metapneumovirus (aMPV) infection in turkeys

Sample Type: Turkey T-lymphocytes

Analysis Method: Flow Cytometry


Dennis Rubbenstroth, Tina S. Dalgaard, Sonja Kothlow, Helle R. Juul-Madsen and Silke Rautenschlein


Developmental & Comparative Immunology (2010) 34, Issue 5, pages 518-529

The avian Metapneumovirus (Ampv) causes an economically important acute respiratoroy disease in turkeys. While antibodies were shown to be insufficient for protection against aMPV-infection, the role of T-lymphocytes in the control aMPV-infection is not clear. In this study, the role of T-lymphocytes in aMPV-pathogenesis in a T-Cell suppression model in turkeys was investigated. Blood samples were collected with a syringe and were immediately transferred to S-Monovette EDTA-tubes. A total volume of 400ul EDTA-treated blood was mixed with 80ul fixation reagent TransFix, resulting in a 1.2-FOLD dilution of the sample. Samples were then stored for up to 1 day until further analysis.

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