Basket: 0
Cytomark  >  Resources  >  Bibliography
Bibliography

Bibliography

Human Blood Stabilisation - Circulating Tumour Cells

2016

KEY PAPER: Detection and Characterization of Circulating Tumor Associated Cells in Metastatic Breast Cancer

Sample Type: CTCs, circulating tumour cell clusters (CTC clusters), CTCs of epithelial-mesenchymal transition (EMT) and cancer associated macrophage-like cells (CAMLs).

Analysis Method: Immunofluorescence staining, Cell sorting, Sanger sequencing

Authors

Z. Mu, N. Benali-Furet, G. Uzan, A. Znaty, Z. Ye, C. Paolillo, C. Wang, L. Austin, G. Rossi, P. Fortina, H. Yang, M. Cristofanilli

Publication

Int. J. Mol. Sci (2016) 17(10): 1665

This paper describes the use of the Screencell method of size-exclusion isolation of CTCs, circulating tumour cell clusters (CTC clusters), CTCs of epithelial-mesenchymal transition (EMT) and cancer associated macrophage-like cells (CAMLs). Blood samples collected in TransFix tubes and analysed within 72 hours. Cytological Analysis was performed by immuno?uorescence staining of cytokeratins (CK-8, 18 and 19), DAPI, CD45 for CTCs, and EMT-CTCs were stained for Vimentin and N-Cadherin. Cell sorting was performed by the DEPArray™ system. TP53 and ESR1 Mutations in CTCs were analysed by Sanger sequencing.

http://www.mdpi.com/1422-0067/17/10/1665/html

High-resolution imaging for the detection and characterisation of circulating tumour cells from patients with oesophageal, hepatocellular, thyroid and ovarian cancers

Sample Type: Circulating tumour cells

Analysis Method:  imaging flow cytometer

Authors

B. M. Dent, L. F. Ogle, R. L. O'Donnell, N. Hayes, U. Malik, N. J. Curtin, A. V. Boddy, E. R. Plummer, R. J. Edmondson, H. L. Reeves, F. E. B. May, D. Jamieson

Publication

International Journal of Cancer (2016) 138(1): 206-216

The purpose of this study was to develop and validate a novel, widely applicable method for detection and characterisation of circulating tumour cells from 4 tumour types. Blood samples were collected in TransFix collection tubes and stained for immunofluorescence by the following membrane antibodies and nuclear stains: EpCAM, cytokeratins 4, 5, 6, 8, 10, 13 and 18, survivin and CD45, and DAPI or DRAQ5. Circulating tumour cells were enriched using an EasySep human CD45 depletion kit, and were analysed using an ImageStream X Flow cytometer.

http://onlinelibrary.wiley.com/doi/10.1002/ijc.29680/full

2015

KEY PAPER: A Novel Strategy for Detection and Enumeration of Circulating Rare Cell Populations in Metastatic Cancer Patients Using Automated Microfluidic Filtration and Multiplex Immunoassay

Sample Type: Circulating rare cells (CTCs, CMCs, CECs, CSCs)

Analysis Method: Automated microfluidic filtration and multiplex immunoassay

Authors

M. J. M. Magbanua, M. Pugia, J. S. Lee, M. Jabon, V. Wang, M. Gubens, K. Marfurt, J. Pence, H. Sidhu, A. Uzgiris, H. S. Rugo, J. W. Park

Publication

PLoS ONE 10(10): e0141166

In this study, the performance of a novel approach for detection and enumeration of multiple rare cell populations was evaluated in the blood of metastatic breast and lung cancer patients using an automated microfluidic filtration and multiplex immunoassay strategy. Different circulating rare cell populations were detected and enumerated, including circulating tumour cells (CTCs), circulating mesenchymal cells (CMCs), circulating endothelial cells (CECs), and putative circulating stem cells (CSCs). Simultaneous assessment of CTCs, CMCs, CSCs and CECs may provide new tools to study mechanisms of disease progression and treatment response/resistance.
The highly controlled filtration process and the multi-step staining parameters were optimised to minimise the detection of false positives in healthy donor blood. Use of TransFix®, along with controlled shipping and storage conditions contributed to the high rate of reportable results (98%).
Blood was collected into tubes containing K3EDTA and 0.45mL Transfix® in customised TransFix/EDTA Vacuum Blood Collection Tubes (TVT-09-50-45).

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0141166

Development of a method to measure acetylated histone H4 in the nuclei of circulating myeloid cells as a surrogate tissue for the pharmacodynamics of HDAC inhibitors in the treatment of solid tumors

Sample Type: Circulating myeloid cells

Analysis Method:  imaging flow cytometer

Authors

J. David, W. Wong, G. Veal

Publication

AACR Cancer Res (2015) 75(15 Suppl):Abstract nr 5374

This conference abstract details the development and validation of an assay to measure the proportion of myeloid cells in peripheral blood with nuclei localised acetylated Histone H4 detectable by immunofluorescence and imaging flow cytometry. Blood samples treated with the potential anti­cancer agent sodium valproate were then stabilised in TransFix, stained by anti­acH4 and DAPI then analsysed by an Imagestream MkII imaging flow cytometer.

http://cancerres.aacrjournals.org/content/75/15_Supplement/5374.short

2013

4EVER: Assessment of circulating tumor cells with a novel, filtration-based method, in a phase IIIb multicenter study for postmenopausal, HER2-negative, estrogen receptor-positive, advanced breast cancer patients

Sample Type: Circulating tumour cells

Analysis Method: Fluorescence scanning microscopy

Authors

P. A. Fasching et al

Publication

J Clin Oncol 31, 2013 (suppl; abstract 591)

The presence of circulating tumor cells (CTC’s) has been shown to be of diagnostic relevance for patients with early and advanced breast cancer (BC) The usefulness of CTC assessments depends upon accurate cell counts and the corresponding analysis of molecular targets. Patient blood samples were preserved using TransFix vacuum blood collection tubes and CTC’s were detected by image analytics after fluorescence scanning microscopy. The clinical trial is ongoing.

http://meetinglibrary.asco.org/content/117629-132


back to top