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Bibliography

Bibliography

2000

The Use of Blood Controls

Reduction of intra and inter laboratory variation in CD34+ stem cell enumeration using stable test material, standard protocols and targeted training

Authors

D.Barnett, V.Granger, J.Kraan, J.T.Reilly, S.Papa & J.W.Gratama

Publication

British Journal of Haematology (2000) 108: 784-792

The European Working Group on Clinical Cell Analysis has developed a single platform flow cytometric protocol for the enumeration of CD34+ stem cells. A standard protocol was used over 24 clinical sites together using a CD34+ stabilised whole blood control. The use of a common standardisation protocol and targeted training significantly reduced intra and inter laboratory CD34+ cell count variation.

http://www.ncbi.nlm.nih.gov/pubmed/10792284

Standardization of lymphocyte antibody binding capacity a multi centre study

Authors

D.Barnett, I.Storie, V.Granger, L.Whitbey, J.T.Reilly, S.Brough, S.Garner, J.Lawry, S.Richards, A.E.Bell and B.K.Shenton

Publication

Clinical Laboratory Haematology (2000) 22: 89-96

Quantitative flow cytometry is increasingly being used to characterise non-malignant and malignant disorders, inter and intra laboratory standardisation becomes an important issue. However the lack of standardisation methods and process controls with defined antibody binding values, limits direct comparison. The study showed that a standard protocol is required to achieve low CVs. Also that stable whole blood can be used as a process control with defined antibody binding capacity values.

http://www.ncbi.nlm.nih.gov/pubmed/10792398


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