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Bibliography

Bibliography

2001

Human Blood Stabilisation - Immunophenotyping

Affordable CD4+ T cell counts by flow cytometry. II. The use of fixed whole blood in resource poor settings

Sample Type: CD4+ cells

Analysis Method: Flow Cytometry

Authors

I. Jani, G.Janossy, A.Iqbal, F. Mhalu, E.Lyamuya, G.Biberfield, D.Glencross, L.Scott, J.T.Reilly, V.Granger & D.Barnett

Publication

Journal of Immunological Methods (2001) 257: 145-154

The feasibility and precision of CD4 counting in resource poor areas was tested using TransFix. It has been shown that immunological gating, including the primary identification of T, B and NK cells by virtue of their specific antibody binding capacity, is a robust method for absolute cell counting. With TransFixed samples primary CD4+ gating can be performed with great precision. There was also no difference in the precision of CD4+ counts performed on fresh cells in Tanzania and TransFixed cells in South Africa. TransFixed samples from smaller clinics can be batched over a period of one week for shipment to local or regional hospitals for analysis.

http://www.ncbi.nlm.nih.gov/pubmed/11687248

CD45 assisted panleucogating for accurate, cost effective dual platform CD4+ T cell enumeration

Sample Type: CD4+ cells

Analysis Method: Flow Cytometry

Authors

Deborah Glencross, Lesley E. Scott, Ilesh V. Jani, David Barnett and George Janossy

Publication

Cytometry (2001) 50: 69-77

A higher accuracy and precision of CD4 counts was documented with Pan Leucogating compared with lymphocyte gating using stabilised blood controls provided by UK NEQAS. These results were verified with 183 fresh samples and 112 TransFixed samples. These observations showed that dual platform leucocyte counts should replace lymphocyte counts.

http://onlinelibrary.wiley.com/doi/10.1002/cyto.10068/abstract

The Use of Blood Controls

Low level leucocyte counting: a critical variable in the validation of leucodepleted blood transfusion components as highlighted by an external quality assurance study

Authors

D. Barnett, K. Goodfellow, J. Ginnever, V. Granger, L. Whitby & J.T. Reilly

Publication

Clinical Laboratory Haematology (2001) 23: 43-51

Leucocyte counts of <5 x 106 per blood transfusion product are currently recommended in the UK in order to reduce transfusion related infections and febrile reactions. Routine leucocyte depletion requires the development of reliable internal and external quality assurance programmes. A stabilised low leucocyte blood control manufactured by UK NEQAS was used to determine inter and intra laboratory CVs. The study highlighted the variability in low level leucocyte counting, especially within the critical range of 5-30 cells/ul. The results highlighted the need for a standardised protocol and nuclear staining reagent for the routine validation of leucocyte depleted blood products.

http://www.ncbi.nlm.nih.gov/pubmed/11422230


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