Basket: 0
Cytomark  >  Resources  >  Bibliography
Bibliography

Bibliography

2003

Human Blood Stabilisation - Immunophenotyping

Monitoring of human immunodeficiency virus infection in resource-constrained countries

Sample Type: CD4+ cells

Analysis Method: Flow Cytometry

Authors

S Crowe, S Turnbull and R Oelrichs

Publication

Clinical Infectious Diseases 37 (Supplement 1): S25-S35. 2003

The reference standards used to monitor human immunodeficiency virus (HIV) infection are flow cytometric analysis of T lymphocyte subsets to provide the CD4+ T cell count and molecular assays to quantify plasma HIV load. Few laboratories in resource-constrained countries can afford to perform these tests. This review discusses the above assays and their role in addition to clinical monitoring in resource-constrained countries. It states that TransFix TM stabilises whole blood for CD4 cell analysis for 7 days at 37 degrees Celsius and <3 days at 42 degrees Celsius. This stabilisation allows the transport of samples from distant sites to laboratories.

http://cid.oxfordjournals.org/content/37/Supplement_1/S25.short

New trends in affordable CD4+ T-cell enumeration by flow cytometry in HIV/AIDS

Sample Type: CD4+ cells

Analysis Method: Flow Cytometry

Authors

Janossy G, Jania IV, Brandob B

Publication

Clinical & Applied Immunology (2003) 4: 91-107

Inexpensive Antiretroviral Therapy (ART) might soon be available to treat human immunodeficiency Virus (HIV) infections in resource-restricted areas of the globe. The number of CD4+ T-cells in the blood is the single most important laboratory parameter to select patients for therapy at the right time and to monitor the effect of ART. Stabilised blood cell standards and blood stabilising fluid, referred to as TransFix have recently been introduced for Quality Assurance. As the fast changing scenery of Flow Cytometry diminished the influence of company-orientated and instrument-orientated Quality Assurance Schemes, more emphasis had to be paid to the use of stabilised standard biological samples in international Quality Assurance Schemes. Preservative fluids, such as TransFix have been introduced with two beneficial effects. Firstly, TransFix added to whole blood at 1:10 dilution stabilises whole blood to perform reliable lymphocyte subset analysis for as long at 10 days. The catchment areas for laboratories can therefore be extended to receive samples from further afield. Secondly. TransFix has an additional bonus for quality assurance and interlaboratory technical standardisation. Samples shipped and tested within the time limits of 10 days between continents can be fully utilised to check the performance of instruments, reagents, full technologies and other parameters.

http://www.sciencedirect.com/science/article/pii/S1529104903000497


back to top