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CSF Stabilisation

Identification of Neoplastic Infiltration of the Cerebrospinal Fluid (CSF) in Patients with Aggressive B-Cell Non-Hodgkin’s Lymphoma (B-NHL) without Clinical Evidence of Leptomeningeal Disease: A Comparative Analysis of the Utility of Flow Cytometry (FCM) Versus Conventional Cytology (CC)

Sample Type: CSF (neoplastic cells, B-NHL)

Analysis Method: Flow Cytometry


Orfao A, Quijano S, Lopez A, Deben G, Salar A, Poderos C, Sancho JM, Vallejo C, Arias J, Garcia JA, Capote F, Morado M, Nicolas C, Briones J, Fernandez S,Carmona, L. Vazquez, and J.F. San Miguel


Blood (ASH Annual Meeting Abstracts) (2006) 108: 4605.

Quote: In all cases, the CSF samples were analysed simultaneously by CC at the institution of origin and FCM, centrally one institution. For the FCM analysis of the CSF, stabilised samples (TransFix, CYTOMARK) were systematically stained with the following combination of monoclonal antibodies: CD8-sIgl/CD56-sIgk/CD4-CD19/CD3/CD20/CD45 (FITC/PE/PERCPCY5.5/PECY7/APC/APCCY7). If the FCM test showed infiltration, an additional 6-color antibody panel was used for full phenotypic characterisation of the disease.

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