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Bibliography

Bibliography

2009

Human Blood Stabilisation - Immunophenotyping

Downregulation of the T-Cell Receptor by Human Immunodeficiency Virus Type 2 Nef Does Not Protect against Disease Progression

Sample Type: T-cells

Analysis Method: Flow Cytometry

Authors

Jérôme Feldmann, Aleksandra Leligdowicz, Assan Jaye, Tao Dong, Hilton Whittle, and Sarah L. Rowland-Jones

Publication

Journal of Virology (2009) 83(24): 12968-12972

T-cell activation marker expression. Fresh whole blood was stabilized in a 5:1 ratio with TransFix (Cytomark) for 2 to 14 days and used for determination of T-cell surface activation marker expression using anti-HLA-DR-fluorescein isothiocyanate-, CD38-phycoerythrin (PE)-, CD4-peridinin chlorophyll protein-, and CD8-allophycocyanin-titrated monoclonal antibodies (BD Pharmingen).

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2786859/

KEY PAPER: Method validation and immunophenotypical study of NK and T lymphocytes by flow cytometry

Sample Type: NK and NKT cells

Analysis Method: Flow Cytometry

Authors

E. Konsta, E. Kwvota

Publication

National and Kapodistrian University of Athens

The purpose of this study was the clarification of the importance of the phenotypic markers oh human NK and NKT cells definition in subjects with a significant increase of these cells percentage. Specimens with malignant populations (B-Chronic Lymphocytic Leukaemia, Acute Myeloblastic Leukaemia and Myelodysplastic Syndrome) were studied in order to evaluate the suitability of a blood stabilising reagent (TransFix) in routine laboratory practise. The study indicates that TransFix can be used reliably in flow cytometric analysis – light scatter characteristics and immunophenotype are well preserved in TransFix-treated specimens stored at 4-10 degrees Celsius, particularly concerning the blasts and lymphocyte populations for 15 days.

http://phdtheses.ekt.gr/eadd/handle/10442/26992

Method Validation and Study of Immunophenotype NK and T lymphocytes by flow cytometry

Sample Type: NK and NKT malignant populations (B-Chronic Lymphocytic Leukaemia, Acute Myeloblastic Leukaemia and Myelodysplastic Syndrome)

Analysis Method: Flow Cytometry

Authors

E. P. Konstas

Publication

PhD Thesis (2009) National and Kapodistrian University of Athens (NCAA)

The purpose of this study was to clarify the importance of the phenotypic markers of human NK and NKT cells in subjects with a significant increase of these cells. One of the aims was to study subjects with malignant populations (B-Chronic Lymphocytic Leukaemia, Acute Myeloblastic Leukaemia and Myelodysplastic Syndrome) in order to evaluate the suitability of a blood stabilizing reagent (TransFix®) in routine laboratory practice. The Author found that TransFix® stabilising reagent can be used reliably in flow cytometric analysis; light scatter characteristics and immunophenotype are well preserved in TransFix®-treated specimens stored at 4-10?C particularly concerning the blasts and lymphocytes populations for 15 days.

https://phdtheses.ekt.gr/eadd/handle/10442/26992

CSF Stabilisation

Identification of Leptomeningeal Disease in Aggressive B-Cell Non-Hodgkin's Lymphoma: Improved Sensitivity of Flow Cytometry

Sample Type: CSF neoplastic cells from B-NHL

Analysis Method: Flow Cytometry

Authors

Quijano S., López A, Sancho JM, Panizo C, Debén G, Castilla C, García-Vela JA, Salar A, Alonso-Vence N, González-Barca J, Peñalver FJ, Plaza-Villa J,Morado M, José García-Marco, Jesús Arias, Javier Briones, Secundino Ferrer, Javier Capote, Concepción Nicolás, and Alberto Orfao

Publication

Journal of Clinical Oncology (2009) 27: 1462 - 1469.

We evaluate the sensitivity and specificity of a new 11-parameter Flow cytometry (FCM) approach versus conventional cytology (CC) for detecting neoplastic cells in stabilized CSF samples from newly diagnosed aggressive B-cell non-Hodgkin’s lymphoma (B-NHL) at high risk of CNS relapse, using a prospective, multicentric study design.

For multi-parameter FCM analyses, CSF samples (median volume, 2.0mL; range, 0.5 to 4.0 mL) were directly collected into tubes containing EDTA and 0.2 mL of TransFix (Cytomark) and shipped overnight to the central FCM laboratory. Immediately on arrival at the central laboratory, the volume of the CSF sample was measured (after subtracting 0.2 mL corresponding to the TransFix solution) and recorded;

http://www.ncbi.nlm.nih.gov/pubmed/19224854


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