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Bibliography

Bibliography

2010

Human Blood Stabilisation - Immunophenotyping

Direct Relationship between Virus Load and Systemic Immune Activation in HIV-2 Infection

Sample Type: T cell activation markers

Analysis Method: Flow Cytometry

Authors

A. Leligdowicz, J. Feldmann, A. Jaye, M. Cotton, T. Dong, A. McMichael, H. Whittle, S. Rowland-Jones

Publication

J Infect Dis (2010) 201 (1): 114-122

This paper reports a study into the relationship between virus load and systemic immune activation in HIV 2 infection. TransFix was used to stabilise whole blood for 2-14 days and used for determination of T cell surface activation marker expression with anti-HLA-DR, CD38, CD4, and CD8.

https://academic.oup.com/jid/article/201/1/114/870467/Direct-Relationship-between-Virus-Load-and

KEY PAPER: Flow cytometric profiles, biomolecular and morphological aspects of transfixed leukocytes and red cells

Sample Type: Lymphomonocytic cells (blood)

Analysis Method: SEM, TEM, Western blotting, and ?ow cytometry (biomolecular and morphological aspects), FC, Tunel, and electrophoresis (DNA behavior)

Authors

Canonico B, Betti M, Luchetti F, Battistelli M, Falcieri E, Ferri P, Zamai L, Barnett D, Papa S.

Publication

Cytometry B, Clinical Cytometry. 2010 July: 78(4): 267-78

Cytometric performance is suboptimal in aged unfixed specimens because of apoptosis that affects light scatter properties. Our findings highlight that lymphomonocytic cells are well stabilized even at suboptimal temperature and cell density. TransFix is able to abolish any apoptotic features and acts as an optimal blood preservative for appropriate preanalytical stabilization.

http://onlinelibrary.wiley.com/doi/10.1002/cyto.b.20510/full

Interlaboratory variability of CD34+ stem cell enumeration. A pilot study to national external quality assessment within the Czech Republic

Sample Type: CD34+ stem cells

Analysis Method: Flow Cytometry

Authors

D. LYSÁK, T. KALINA, J. MARTÍNEK, Z. PIKALOVÁ, D. VOKURKOVÁ, M. JAREŠOVÁ, I. MARINOV, A. ONDREJKOVÁ, M. ŠPACEK, O. STEHLÍKOVÁ

Publication

International Journal of Laboratory Hematology (2010) 32, e229-e236

Quote: The study took place between November 2007 and May 2009 and consisted of three surveys. Ten laboratories took part in the study, but only nine laboratories participated in the first survey. Each laboratory was assigned a unique number (ULN) to retain confidentiality. Two samples of mobilized peripheral blood were included in every survey. Samples were stabilized with TransFix (Cytomark Ltd, Buckingham, UK),divided in 1 ml aliquots and shipped overnight by courier to the participants. The blood was collected from patients or healthy donors who underwent stem cell mobilization after giving informed consent. All subjects were negative for infectious diseases (humanimmunodeficiency virus, hepatitis B and C, syphilis).The stability of the samples was assured for at least 3 days (CV up to 5% in the stability study).Each laboratory performed the CD34+ enumeration according to its local protocol and practice and reported the measured values (leukocyte count,CD34+ percentage and absolute count) and some methodological details. All data were analyzed in the co-ordinating laboratory, and the results sent to the participants. The absolute CD34+ count was assignedas an indicator of laboratory performance.

http://www.ncbi.nlm.nih.gov/pubmed/20561093

CSF Stabilisation

Clinical significance of occult cerebrospinal fluid involvement assessed by flow cytometry in non-Hodgkin's lymphoma patients at high risk of central nervous system

Sample Type: CSF (non-Hodgkin's lymphoma)

Analysis Method: Flow Cytometry

Authors

Juan-Manuel Sancho, Alberto Orfao, Sandra Quijano, Olga García, Carlos Panizo, Elena Pérez-Ceballos, Guillermo Deben, Antonio Salar, Eva González-Barca, Natalia Alonso, Jose-Antonio García-Vela, Javier Capote, Francisco-Javier Peńalver, Mariano Provencio, Jesús Arias, Josefa Plaza, Dolores Caballero, Marta Morado, Evarist Feliu, Josep-Maria Ribera

Publication

European Journal of Haematology (2010) 85, Issue 4, 321–328

Quote: The specific techniques and procedures used for the analyses of CSF samples have been previously described in detail (21). CSF samples were analysed in parallel using CC in the hospital of origin and centrally by FCM at the Cytometry Service of the University of Salamanca (Spain) using a standardised 11-parameter FCM immunophenotypic assay of CSF samples stabilised with 0.2 mL of TransFix added at the hospital of origin.

http://www.ncbi.nlm.nih.gov/pubmed/20528905

Animal Blood Stabilisation

KEY PAPER: Effects of Cyclosporin A induced T-lymphocyte depletion on the course of avian Metapneumovirus (aMPV) infection in turkeys

Sample Type: Turkey T-lymphocytes

Analysis Method: Flow Cytometry

Authors

Dennis Rubbenstroth, Tina S. Dalgaard, Sonja Kothlow, Helle R. Juul-Madsen and Silke Rautenschlein

Publication

Developmental & Comparative Immunology (2010) 34, Issue 5, pages 518-529

The avian Metapneumovirus (Ampv) causes an economically important acute respiratoroy disease in turkeys. While antibodies were shown to be insufficient for protection against aMPV-infection, the role of T-lymphocytes in the control aMPV-infection is not clear. In this study, the role of T-lymphocytes in aMPV-pathogenesis in a T-Cell suppression model in turkeys was investigated. Blood samples were collected with a syringe and were immediately transferred to S-Monovette EDTA-tubes. A total volume of 400ul EDTA-treated blood was mixed with 80ul fixation reagent TransFix, resulting in a 1.2-FOLD dilution of the sample. Samples were then stored for up to 1 day until further analysis.


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