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Bibliography

Bibliography

2012

Human Blood Stabilisation - Immunophenotyping

TransFix for delayed flow cytometry of endothelial progenitor cells and angiogenic T cells

Sample Type: Endothelial progenitor cells (EPC) and angiogenic T cells

Analysis Method: Flow Cytometry

Authors

V. Y. Hoymans, A. H. Van Craenenbroeck, L.Bruyndonckx, S. H. van Ierssel, C .J. Vrints, V. M. Conraads, E. M. Van Craenenbroeck

Publication

Microvascular Research, Volume 84, Issue 3, November 2012, Pages 384–386

Endothelial progenitor cells (EPC) and angiogenic T cells have not been validated for use in studies that involve delayed sample processing and analysis. Here, we report our results for the flow cytometric enumeration of circulating EPC and angiogenic T cells using TransFix-treated whole blood obtained from adult patients with cardiovascular disease and healthy volunteers. Both cell types promote neovascularization and vascular homeostasis. As such they have been put forward as novel diagnostic markers for endothelial dysfunction and may add prognostic information in patients with cardiovascular disease. Our findings indicate that by the addition of TransFix cellular antigen stabilizing reagent to whole blood, analyses can be postponed up to 7 days after blood collection. Therefore, this procedure may facilitate laboratory workflow, as well as the organization of multicenter studies, which requires analyses to be conducted in a central core laboratory.

http://www.sciencedirect.com/science/article/pii/S0026286212001562

CSF Stabilisation

Clinical relevance of flow cytometric immunophenotyping of the cerebrospinal fluid in patients with diffuse large B-cell lymphoma

Sample Type: CSF (large B cell lymphoma)

Analysis Method: Flow Cytometry

Authors

R. Alvarez, J. Dupuis, 1, A. Plonquet, C. Christov, C. Copie-Bergman, F. Hemery, I. Gaillard, T. El Gnaoui, F. Kuhnowski, M. Bedoui, K. Belhadj, P. Brugières and C. Haioun

Publication

Annals of Oncology, Volume 23, Issue 5 , pages 1274-1279, 2012

Central nervous system (CNS) relapse is an uncommon but dramatic complication of diffuse large B-cell lymphoma (DLBCL). Several studies have demonstrated the superiority of cerebrospinal fluid (CSF) flow cytometry (FCM), as compared with conventional cytology (CC), in detecting occult leptomeningeal disease. The clinical relevance of a positive FCM still has to be clarified. Makes reference to use of TransFix to stabilise and transport CSF samples.

http://annonc.oxfordjournals.org/content/23/5/1274.short

Current strategies in the diagnosis of diffuse large B-cell lymphoma of the central nervous system

Sample Type: CSF (large B cell lymphoma)

Analysis Method: Flow Cytometry

Authors

Alexander Baraniskin, Martina Deckert, Gernot Schulte-Altedorneburg, Uwe Schlegel, Roland Schroers

Publication

British Journal of Haematology, Volume 156, Issue 4, pages 421–432, February 2012

A detailed review of current strategies used in the diagnosis of diffuse large B cell lymphoma. The use of flow cytometry to detect CSF involvement in Primary CNS Lymphoma (PCNSL )provides improved diagnostic sensitivity as compared to cytopathology alone. With the availability of TransFix it is now possible to preserve CSF for up to 10 days. Accordingly it is now possible to analyse CSF samples from PCNSL patients in a central laboratory, preferably within a large multi-centre study.

http://onlinelibrary.wiley.com/doi/10.1111/bjh.2012.156.issue-4/issuetoc

EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes

Sample Type: CSF and Vitreous biopsy

Analysis Method: Flow Cytometry

Authors

J. J. M. van Dongen, L. Lhermitte, S. Böttcher, J. Almeida, V. H. J. van der Velden, J. Flores-Montero, A. Rawstron, V. Asnafi, Q. Lécrevisse, P. Lucio, E. Mejstrikova, T. Szczepański, T Kalina, R. de Tute, M. Brüggemann, L Sedek, M. Cullen, A. W. Langerak, A. Mendonça, E. Macintyre, M. Martin-Ayuso, O. Hrusak, M. B. Vidriales, A. Orfao

Publication

Leukemia (2012) 26: 1908?1975

Section 3 discusses the design and evaluation of the 8-colour, 13 parameter Euroflow™ small sample tube (SST) and related sample preparation protocols aimed to evaluate CSF and Vitreous biopsy samples, in patients suspected of leukaemia. Samples collected either with culture medium with FBS of FCS, or in TransFix Sample Storage Tubes, which the author states in the general discussion ?result in optimal stabilization of the cells for a few days (for example, 48h) and improve cell detection?.

https://www.nature.com/leu/journal/v26/n9/pdf/leu2012120a.pdf

Flow cytometry as a diagnostic tool in lymphomatous or leukemic meningitis

Sample Type: CSF (NM)

Analysis Method: Flow Cytometry

Authors

M.S. Ahluwalia, P.K. Wallace and D.M. Peereboom

Publication

Cancer, Volume 118, Issue 7, pages 1747 – 1753, 2012

In patients with neoplastic meningitis (NM), early diagnosis is highly desirable because the rapid institution of intrathecal therapy may mitigate the course of the disease. Cytology, long considered the "gold standard" for diagnosis, has low sensitivity because of both the paucity of cells in the cerebrospinal fluid (CSF) and morphological similarities between benign and malignant cells. A comprehensive review of the literature from 2005 through 2011 was performed that focused on diagnostic modalities for lymphomatous meningitis. Several studies demonstrated the sensitivity of flow cytometry to be several-fold higher than that of cytology for the detection of CSF leukemia/lymphoma. Makes reference to use of TransFix to stabilise and transport CSF samples.

http://onlinelibrary.wiley.com/doi/10.1002/cncr.26335/full

Role of flow cytometry immunophenotyping in the diagnosis of leptomeningeal carcinomatosis

Sample Type: CSF (leptomeningeal carcinomatosis)

Analysis Method: Flow Cytometry

Authors

D. Subirá, C. Serrano, S. Castañón, R. Gonzalo, J. Illán, J. Pardo, M. Martínez-García, E. Millastre, F. Aparisi, M. Navarro, M. Dómine, I. Gil-Bazo, P. Pérez Segura, M. Gil and J. Bruna

Publication

Neuro-Oncology, Volume 14, Issue 1, pages 43-52, 2012

Purpose: To explore the contribution of flow cytometry immunophenotyping (FCI) in detecting leptomeningeal disease in patients with solid tumors. CSF samples obtained from a lumbar puncture (n = 77) and from an Ommaya reservoir (n = 1) were collected in EDTA tubes containing 0.2 mL of an immunofixative reagent (TransFix, Cytomark), necessary to guarantee safe transportation. FCI seems to be a promising new tool for improving the diagnostic examination of patients with suspicion of LC. Detection of epithelial cells with a higher DNA content is highly specific of LC, but evaluation of the nonepithelial cell compartment of the CSF might also be useful for supporting this diagnosis.

http://neuro-oncology.oxfordjournals.org/content/14/1/43.short

Animal Blood Stabilisation

KEY PAPER: A rapid high-precision flow cytometry based technique for total white blood cell counting in chickens

Sample Type: Chicken White Blood Cell

Analysis Method: Flow Cytometry

Authors

C.Seliger, B. Schaerer, M. Kohn, H. Pendl, S. Weigend, B.Kaspers, S. Härtle

Publication

Veterinary Immunology and Immunopathology, Volume 145, pages 86–99, 2012

TransFix (Cytomark) was used to stabilise samples to demonstrate that samples can be shipped and stored prior to analysis. TransFix was used to stabilise and transport the avian blood samples. The automated analysis of total white blood cell count and white blood cell differentials is routine in research and clinical diagnosis in mammalian species. In contrast, in avian haematology these parameters are still estimated by conventional microscopic procedures due to technical difficulties associated with the morphological peculiarities of avian erythrocytes and thrombocytes. Both cell types are nucleated and fairly resistant to cell lysis, a prerequisite for automated leukocyte quantification and differentiation by commercial instruments. By using an anti-CD45 monoclonal antibody in combination with selected subset specific markers we have established a simple (no-lyse no-wash single-step one-tube) flow cytometry based technique for high precision chicken blood cell quantification.

http://www.sciencedirect.com/science/article/pii/S016524271100417X

Other Sample Types

Effects of nano-TiO 2 aerosols showing two distinct agglomeration states on rat lungs

Sample Type: Bronchoalveolar lavage fluid

Analysis Method: Haemocytometer, cytospin, Bio-Plex Luminex assay

Authors

A Noel, K Maghni, C Dion, KJ Wilkinson, S. Hallé, R. Tardif, G. Truchon

Publication

Toxicology Letters, Volume 214, Issue 2, 17 October 2012, Pages 109–119

The study examined inflammation resulting from the inhalation of nono-Ti02 aerosols by rats. After exposure to inhalation the rats were anaesthetized with isoflurane and sacrificed by exsanguination. Fluids from the BAL (BALF) were collected with 0.9% saline. The lungs were washed with a total of 21ml of 0.9 % PBS in 5 steps. These samples were centrifuged to isolate the cells and the cells from the five washes combined. The cell pellet was re-suspended in 1ml of 0.9% PBS containing 1% BSA and 200ul of TransFix. TransFix stabilized cell fractions were examined using a range of techniques to determine the degree of inflammation resulting from inhalation of nano-Ti2 aerosols. The techniques used to examine the TransFix stabilized leucocytes included; total cell counts using a haemocytometer, differential cell counts for lymphocytes using the cytospin cell staining method and inflammation markers were quantified (IL-1 , IL-6, TNF-, MIP-1 and MCP-1) using the Bio-Plex Luminex assay.

http://www.sciencedirect.com/science/article/pii/S0378427412012647


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