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Bibliography

Bibliography

2013

Human Blood Stabilisation - Circulating Tumour Cells

4EVER: Assessment of circulating tumor cells with a novel, filtration-based method, in a phase IIIb multicenter study for postmenopausal, HER2-negative, estrogen receptor-positive, advanced breast cancer patients

Sample Type: Circulating tumour cells

Analysis Method: Fluorescence scanning microscopy

Authors

P. A. Fasching et al

Publication

J Clin Oncol 31, 2013 (suppl; abstract 591)

The presence of circulating tumor cells (CTC’s) has been shown to be of diagnostic relevance for patients with early and advanced breast cancer (BC) The usefulness of CTC assessments depends upon accurate cell counts and the corresponding analysis of molecular targets. Patient blood samples were preserved using TransFix vacuum blood collection tubes and CTC’s were detected by image analytics after fluorescence scanning microscopy. The clinical trial is ongoing.

http://meetinglibrary.asco.org/content/117629-132

Human Blood Stabilisation - Other

Quantification of circulating CD34+/KDR+/CD45 dim endothelial progenitor cells: analytical considerations

Sample Type: Circulating endothelial progenitor cells

Analysis Method: Flow Cytometry

Authors

E. M. Van Craenenbroeck, A. H. Van Craenenbroeck, S. van Ierssel, L. Bruyndonckx, V. Y. Hoymans, C J. Vrints, V. M. Conraads

Publication

International Journal of Cardiology, 167 (5): 1688-1695

A comprehensive review of characterisation of circulating endothelial progenitor cells with a particular focus on the considerations surrounding enumeration by flow cytometry and their impact on cardiovascular disease. The authors note TransFix can be used to prolong sample storage time for 7 days for this type of analysis.

http://www.sciencedirect.com/science/article/pii/S0167527312014222

Relationships between emerging cardiovascular risk factors, z-BMI, waist circumference and body adiposity index (BAI) on adolescents

Sample Type: Circulating endothelial cells

Analysis Method: Flow Cytometry

Authors

I. B. F. Dias, D. G. Panazzolo,M. F. Marques, B. D. Paredes, M. G. C. Souza, D. P. Manhanini,V. Morandi, P. T. V. Farinatti, E. Bouskela, L. G. Kraemer-Aguiar

Publication

Clin Endocrinol (2013) 79(5): 667-674

The body adiposity index (BAI) has been recently proposed as an alternative index to body mass index (BMI) and waist circumference (WC) to evaluate adiposity in adults. This study aimed to determine the correlation between BAI and emerging cardiovascular risk markers including circulating endothelial cells (CECs) in normal weight and obese adolescents. Percentage of CECs in peripheral blood were assessed by fow cytometric analysis: 400 uL cellular stabilization solution (TransFix) was added to a 4 ml of peripheral blood to preserve cell surface antigens.

http://onlinelibrary.wiley.com/doi/10.1111/cen.12195/full

CSF Stabilisation

Detection of occult cerebrospinal fluid involvement during maintenance therapy identifies a group of children with acute lymphoblastic leukemia at high risk for relapse

Sample Type: Lymphoblasts in CSF (ALL)

Analysis Method: Flow Cytometry

Authors

C. Martínez-Laperche, A. M. Gómez-García, Á. Lassaletta, C. Moscardó, J. L. Vivanco, J. Molina, J. L. Fuster, J. M. Couselo, J. Sánchez de Toledo, E. Bureo, L. Madero, M. Ramírez

Publication

American Journal of Hematology, 88(5): 359–364, May 2013

To determine the clinical significance of the levels of lymphoblasts in the cerebrospinal fluid (CSF) of children diagnosed with acute lymphoblastic leukaemia (ALL).CSF samples from 136 patients were analysed by conventional cytology (CC) and flow cytometry (FCM) at the time of diagnosis and after intra-thecal therapy (IT). CSF samples for analysis by FCM were treated with TransFix at sample collection to facilitate transport of samples from 8 different hospitals to one centre for analysis. The results support previous findings that FCM analysis was more sensitive than CC in the detection of leukemic cells present in the CSF samples and can provide a powerful tool in detecting residual leukemic cells in the central nervous system.

http://onlinelibrary.wiley.com/doi/10.1002/ajh.23407/full


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