Basket: 0
Cytomark  >  Resources  >  Bibliography
Bibliography

Bibliography

2016

Human Blood Stabilisation - Immunophenotyping

KEY PAPER: First-in-Human Study in Healthy Subjects with FR104, a Pegylated Monoclonal Antibody Fragment Antagonist of CD28

Sample Type: Lymphocyte subsets

Analysis Method: Flow Cytometry

Authors

N. Poirier, G. Blancho, M. Hiance, C. Mary, T. Van Assche, J. Lempoels, S. Ramael, W. Wang, V. Thepenier, C. Braudeau, N. Salabert, R. Josien, I. Anderson, I. Gourley, J. Soulillou, D. Coquoz, B. Vanhove

Publication

J Immunol?DOI: https://doi.org/10.4049/jimmunol.1601538

This paper documents a phase I clinical trial to test FR104 (an antibody which inhibits CD28) in humans as a potential treatment of transplant rejection and autoimmune diseases. Blood samples were collected in TransFix/EDTA Vacuum Blood Collection Tubes (TVTs) to monitor lymphocyte subsets (CD45, CD3, CD28, CD45RO, CD4, CD25, CD127. CD69, CCR7) at time points ranging from day 0 to day 113, from a cohort of 64 healthy subjects.

Phlebotomy time points were initially very frequent post drug administration (0.5, 0.75, 1, 2, 4, 8 and 24 hours). TVTs gave the benefit of providing accurate snapshots of the immune profile of the subjects within this condensed testing period whilst giving the technician the flexibility to test the blood in a more leisurely timescale.

http://www.jimmunol.org/content/early/2016/11/12/jimmunol.1601538.abstract

Human Blood Stabilisation - Circulating Tumour Cells

KEY PAPER: Detection and Characterization of Circulating Tumor Associated Cells in Metastatic Breast Cancer

Sample Type: CTCs, circulating tumour cell clusters (CTC clusters), CTCs of epithelial-mesenchymal transition (EMT) and cancer associated macrophage-like cells (CAMLs).

Analysis Method: Immunofluorescence staining, Cell sorting, Sanger sequencing

Authors

Z. Mu, N. Benali-Furet, G. Uzan, A. Znaty, Z. Ye, C. Paolillo, C. Wang, L. Austin, G. Rossi, P. Fortina, H. Yang, M. Cristofanilli

Publication

Int. J. Mol. Sci (2016) 17(10): 1665

This paper describes the use of the Screencell method of size-exclusion isolation of CTCs, circulating tumour cell clusters (CTC clusters), CTCs of epithelial-mesenchymal transition (EMT) and cancer associated macrophage-like cells (CAMLs). Blood samples collected in TransFix tubes and analysed within 72 hours. Cytological Analysis was performed by immuno?uorescence staining of cytokeratins (CK-8, 18 and 19), DAPI, CD45 for CTCs, and EMT-CTCs were stained for Vimentin and N-Cadherin. Cell sorting was performed by the DEPArray™ system. TP53 and ESR1 Mutations in CTCs were analysed by Sanger sequencing.

http://www.mdpi.com/1422-0067/17/10/1665/html

High-resolution imaging for the detection and characterisation of circulating tumour cells from patients with oesophageal, hepatocellular, thyroid and ovarian cancers

Sample Type: Circulating tumour cells

Analysis Method:  imaging flow cytometer

Authors

B. M. Dent, L. F. Ogle, R. L. O'Donnell, N. Hayes, U. Malik, N. J. Curtin, A. V. Boddy, E. R. Plummer, R. J. Edmondson, H. L. Reeves, F. E. B. May, D. Jamieson

Publication

International Journal of Cancer (2016) 138(1): 206-216

The purpose of this study was to develop and validate a novel, widely applicable method for detection and characterisation of circulating tumour cells from 4 tumour types. Blood samples were collected in TransFix collection tubes and stained for immunofluorescence by the following membrane antibodies and nuclear stains: EpCAM, cytokeratins 4, 5, 6, 8, 10, 13 and 18, survivin and CD45, and DAPI or DRAQ5. Circulating tumour cells were enriched using an EasySep human CD45 depletion kit, and were analysed using an ImageStream X Flow cytometer.

http://onlinelibrary.wiley.com/doi/10.1002/ijc.29680/full

Human Blood Stabilisation - Other

Stabilization of Transfected Cells Expressing Low-Incidence Blood Group Antigens: Novel Methods Facilitating Their Use as Reagent-Cells

Sample Type: Cell lines expressing blood group antigens

Analysis Method: Morphological analysis

Authors

C. González, R. Esteban, C. Canals, E. Muñiz-Díaz, N. Nogués

Publication

PLoS ONE (2016) 11(9): e0161968

This paper aimed to generate cell lines expressing blood group antigens (to represent low incidence blood group phenotypes) and to then stabilise these to facilitate their use as reagent cells in clinical laboratories. The addition of TransFix to the cells allowed cell stabilisation and antigen detection for at least 120 days and may facilitate the use of these cells in clinical laboratories, overcoming the limited availability of rare red blood cells. TransFix treated cells were test at 11 time points from Day 0 up to Day 120 showing better morphological preservation (FSC & SSC) vs fresh than lyophilised cells.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161968

The Association of High Prevalence of Trophozoites in Peripheral Blood with Lower Antibody Response to P. falciparum Infected Erythrocytes among Asymptomatic Children in Sudan

Sample Type: Malaria infected erythrocytes

Analysis Method: Flow Cytometry

Authors

S. N. Mohamed, D. A. Hassan, A. M. El Hussein, I. M. Osman, M. E. Ibrahim, H. S. Mohamed, B. Y. Nour, N. H. Abdulhadi

Publication

Mediators of Inflammation Article ID 7987686

The Authors of this paper examined the antibody reactivity of autologous plasma from symptomatic and asymptomatic malaria infected children against the infected erythrocytes' surface antigens using flow cytometry. The Authors use TransFix to stabilise variant surface antigens (VSA) for flow cytometric analysis.

https://www.hindawi.com/journals/mi/2016/7987686/abs/

CSF Stabilisation

How to facilitate early diagnosis of CNS involvement in malignant lymphoma

Sample Type: CSF (malignant lymphoma)

Analysis Method: Flow Cytometry

Authors

A. Korfel, M. Nowosielski, J. Pardo-Moreno, F. J. Penalver, G. Buda, H. Bennani, M. Costopoulos, M. Le Garff-Tavernier, C. Soussain, M. Schmid, J. A. Orfao, M. Glantz

Publication

Expert Review of Hematology, 9 (11) https://doi.org/10.1080/17474086.2016.1242405

The authors discuss several analysis methods to facilitate early diagnosis of CNS involvement in malignant lymphoma (CD19/10/45/kappa/lambda for B cell Lymphomas). They recommend stabilising CSF with TransFix if samples cannot be analysed within 1 hour for flow cytometric analysis.

http://www.tandfonline.com/doi/abs/10.1080/17474086.2016.1242405

Leukemic blasts are present at low levels in spinal fluid in one-third of childhood acute lymphoblastic leukemia cases

Sample Type: CSF (B-cell precursor ALL and T-cell ALL)

Analysis Method: Flow Cytometry

Authors

M. Levinsen, H. V. Marquart, L. Groth-Pedersen, J. Abrahamsson, B. K. Albertsen, M. K. Andersen, T. L. Frandsen, A. Harila-Saari, C. Pronk, A. Ulvmoen, G. Vaitkevi?ien?, P. M. L?hteenm?ki, R. Niinim?ki, M. Taskinen, M. Jeppesen, K. Schmiegelow

Publication

Pediatr Blood Cancer, 63 (11): 1935-1942

The authors assessed centralised ?ow cytometry of locally TransFix-?xed cerebrospinal ?uid (CSF) samples versus local conventional cytospin-based cytology for detecting leukemic cells and evaluating kinetics of elimination of leukemic cells in CSF. CSF samples were collected into tubes containing TransFix to stabilise B-cell precursor ALL (CD3/CD10/CD19/CD20/CD34/CD38/CD45), and T-cell ALL (CD3/CD4/CD7/CD8/CD45/CD56), allowing samples to be shipped for centralised analysis, preferably within three days of lumbar puncture. To verify leukemic classification, the CSF from 2 patients were flow sorted followed by FISH targeting the leukemic karyotypes. The authors recommend the use of on-site fixation and rapid centralised flow cytometric analysis of CSF samples.

http://onlinelibrary.wiley.com/doi/10.1002/pbc.26128/full

Bone Marrow Stabilisation

The utilisation of modern transport and telecommunications platforms to assist in the remote provision of paediatric cancer diagnostics in Tanzania

Sample Type: Bone marrow

Analysis Method: Flow Cytometry

Authors

M. Mcdermott, K. O'Hare, P. Scanlon, D. Grehan, S. Rooney, R. Laiti, J. Kaijage, S. Jumanne, M. O'Sullivan

Publication

Pediatric Blood and Cancer, 63: Supplement 3 (S71)

Conference Abstract: Interim diagnostic services were provided by OLCHC in Dublin for paediatric cancer screening in Tanzania utilising DHL to transport fixed bone marrow samples and communicating the results via the WhatsApp communication platform in an average of 2.9 days. The bone marrow samples were fixed with TransFix to maintain integrity during transportation.

https://www.cytomark.co.uk/downloads/Mcdermott_2016_(conference_abstract).pdf


back to top