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Bibliography

Bibliography

2017

CSF Stabilisation

Guidelines For Diagnosis, Prevention And Management Of Central Nervous System Involvement In Diffuse Large B-Cell Lymphoma Patients By The Spanish Lymphoma Group (GELTAMO)

Sample Type: CSF (Diffuse Large B-Cell Lymphoma)

Analysis Method: Flow Cytometry

Authors

F. J. PeÑalver, J. M. Sancho, A. de la Fuente, M. T. Olave, A. Martín, C. Panizo, E. Pérez, A. Salar, A. Orfao

Publication

Haematologica (2017) 102: 235-245

This report by the Spanish Lymphoma Group (GELTAMO) aims to provide useful guidelines and recommendations for the prevention, diagnosis, and treatment of central nervous system diffuse large B-cell lymphoma patients with, or at risk of, leptomeningeal and/or brain parenchyma lymphoma relapse. The Authors summarise and recommend the use of standardised and validated >8-colour FCM evaluation of stabilised CSF in the diagnosis work-up of DLBCL patients at risk of CNS disease for the identification of occult CNSL, and state that immediate sample preservation (preferably in TransFix) is strongly recommended.

http://www.haematologica.org/content/102/2/235.full.pdf+html

Higher rate of central nervous system involvement by flow cytometry than morphology in acute lymphoblastic leukemia

Sample Type: CSF (ALL)

Analysis Method: Flow Cytometry

Authors

J. Dass, A. Dayama, P. C. Mishra, M. Mahapatra, T. Seth, S. Tyagi, H. P. Pati, R. Saxena

Publication

Int J Lab Hem.?39: 546-551.?https://doi.org/10.1111/ijlh.12694

This paper investigates the use of flow cytometry (FCM) as apposed to traditional cytopathology methods for diagnosis of central nervous system (CNS) involvement in acute lymphoblastic leukaemia (ALL). In the discussion, the author discusses the limitations of FCM, such as the viability declines rapidly in cerebrospinal fluid (CSF) samples, and cell degeneration occurs within 1 hour of sample collection. As a solution, the author recommends the use of TransFix to stabilise CSF for up to 72 hrs.

http://onlinelibrary.wiley.com/doi/10.1111/ijlh.12694/full

KEY PAPER: Study of body fluid samples using flow cytometry: Six years of experience at the Hospital Universitario San Ignacio -Ponti cia Universidad Javeriana, Bogota - Colombia

Sample Type: Cerebrospinal fluid, bronchoalveolar lavage, pleural fluid, pericardial fluid, ascite fluid

Analysis Method: Flow Cytometry

Authors

A. Campos, L. Trujillo, D. López, L. Beltrán, E. Arias, G. Vélez, A. Infante, I. De Los Reyes, M. Vizcaíno, P. C. Guzmán, M. V. Herrera, J. Solano, D. Londo?o, A. Ca?as, F. Pretelt, J. C. Pérez, C. Cardozo, S. Fiorentino, S. Quijano

Publication

Universitas Scientiarum, 22(2): 123 - 143, 2017. doi: 10.11144/Javeriana.SC22-2.sobf

The Authors discuss findings from 6 years of experience in implementing flow cytometry in Columbia. The authors used TransFix to stabilise all sample, consisting of mainly cerebrospinal fluid, but also bronchoalveolar lavage, pleural fluid, pericardial fluid and ascite fluid from patients with acute and chronic leukaemia, myelodysplastic syndromes, lymphomas, myeloma, autoimmune diseases, immunodeficiencies and solid tumours. The Authors final conclude that this work represents the first report at the national level supporting Transfix implementation in pre-analytical FCM studies of all body fluids that are processed in the clinical practice.

https://search.proquest.com/openview/538464d63f7cc2192885d3559b2871f6/1?pq-origsite=gscholar&cbl=2041198

Ten-color 15-antibody flow cytometry panel for immunophenotyping of lymphocyte population

Sample Type: CSF lymphocytes

Analysis Method: Flow Cytometry

Authors

A. Rajab, O. Axler, J. Leung, M. Wozniak, A. Porwit

Publication

International Society for Laboratory Hematology (2017) 39 (S1): 76-85

The Authors of this paper have developed a lymphoproliferative disorder screening tube (LPD- ST) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD- ST consists of CD4/kappa FITC, CD8/lambda PE, CD3/CD14ECD, CD38PC5.5, CD20/CD56PC7, CD10APC, CD19APC- A700, CD5APC- A750, CD57/CD23PB and CD45KO. They also claim that this new LPD- ST is more suitable with paucicellular samples such as cerebrospinal fluid (CSF) than previous lymphocyte subset panels. In their experiments, the Authors fix CSF samples in TransFix, and it is inferred that flow results match immunohistochemical staining results.

http://onlinelibrary.wiley.com/doi/10.1111/ijlh.12678/full

Animal Blood Stabilisation

Vaccination Against Histomonosis Limits Pronounced Changes of B-cells and T-cell Subsets in Turkeys and Chickens

Sample Type: Chicken and Turkey T-cell and B-cell subsets

Analysis Method: Flow Cytometry

Authors

T. Mitraa, W. Gernerb, F. A. Kidanea, P. Wernsdorfa, M. Hessa, A. Saalmüllerb, D. Liebharta

Publication

Vaccine (2017) 35(33): 4184-4196

The protozoan parasite histomonas is causative agent of histomonosis in Turkeys and chickens and causes a high mortality rate in Turkeys. Experimental vaccination has shown to protect these species. TransFix was used to stabilise 0.75ml and 1ml whole blood samples in order to analyse monocytes/macrophages and heterophils via Flow cytometry.

http://www.sciencedirect.com/science/article/pii/S0264410X17308228


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