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Cytomark  >  Blood Controls  >  Technology and Preparation of CD4 Blood Controls
Blood controls for flow cytometry

Technology and Preparation of CD4 Blood Controls

CD4 stabilised blood controls are prepared using the proprietary CytoFix® blood stabilisation technology. The stabilisation process is robust resulting in controls that are stable for up to 90 days from the date of manufacture when stored at 2 - 8C, and an open vial stability of 1 month.

Two CD4 stabilised blood controls are available; a normal CD4 blood cell profile, refer to graphs A and B below (Product Code: CF04-N) and a low CD4 profile, refer to graphs C and D below (Product Code: CF04-L). These controls can be purchased separately or as a pair (Product Code: CF04-Duo). Each control is 1.5ml in volume, evaluation samples are available, please contact us for details.

In the controls, the white cell profile is adjusted to give a specific CD4+ T cell count. The normal CD4 control (CF04-N) has a CD4+ T cell absolute count within the normal healthy range (between 410 and 1590 cells/l of blood). The low CD4+ control (CF04-L) has a CD4+ T cell absolute count of between 200 and 300 cells/l. Quality control testing is performed using the following method: Stain/enumeration (using BD Multitest IMK kit, and Trucount tubes), lyse (BD FACS Lyse), no-wash and flow cytometric testing on the BD FACSCalibur. All Blood Controls are supplied with a detailed QC certificate detailing the average count and range for T cells, CD4+ T cells, CD8+ T cells, NK cells and B cells. Click here for QC Certificates

CD4 blood controls prepared using the Cytofix® technology can also be used in External Quality Assurance (EQA) programmes due to their stability.

CD4 Normal (CF04-N)

CD4 Normal (CF04-N)

Graphs A) and B) show the typical lymphocytic profile for CD4 Normal Blood Control.

CD4 Low (CF04-L)

CD4 Low (CF04-L)

Graphs C) and D) show the typical lymphocytic profile for a CD4 Low Blood Control, with reduced CD4+ T cells.

Storage and Stability

CD4 Blood Controls are stable for 3 months from the date of manufacture when stored at 2-8°C. Open vial stability is one month. Vials must not be frozen. A certificate of analysis can be provided with every CD4 Blood Controls product.

Safety

All human source material used to manufacture this product was non-reactive for Hepatitis B, negative for antibodies to HIV (HIV-1/HIV-2) and Hepatitis C (HCV), HIV-1 RNA and HCV RNA. However, this product should be handled as if capable of transferring infection and disposed of with the proper precautions.

Product Code   Description
CF04-L   CD4 Low Blood Control
CF04-N   CD4 Normal Blood Control
CF04-Duo   CD4 Low and Normal Blood Control Set

Blood Controls Made to Order

Blood Controls are available with a minimum order volume of 50 packs and will be manufactured on request. Please contact cytomark@caltagmedsystems.co.uk to discuss your requirements.


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